molecular_biology19 ADP Cell lysis buffer, 35% Hydrogene peroxide Molarity for ADP ribosylation immunoblotting ADP ribosylation immunoblotting은 일반적인 단백질 immunoblotting 보다 더 정교한 실험입니다. 정교한 실험인 만큼 cell lysis buffer가 일반적인 RIPA buffer 조성에서 차이가 있습니다. 이 글에서는 cell lysis buffer 조성, 35% hydrogen peroxide를 Molarity로 계산하는 방법에 대해서 설명을 하겠습니다. ADP ribosylation immunoblotting을 준비하시는 분들에게 도움이 되는 정보이길 바랍니다.본 내용의 참고문헌은 다음과 같습니다.1. ADP Cell lysis buffer 조성과 농도BufferStock ConcentrationWorking ConcentrationVolume (5ml)SDS10%.. 2024. 9. 10. Unlocking Cancer's Code: An In-depth Exploration of MHC antigen presentation, Neoantigens, and Tumor Evolution in the Context of Immune Checkpoint Blockade Explore the heart of immunotherapy, delving into MHC antigen presentation, neoantigens, and tumor evolution. Discover the role of positive and negative selection in shaping tumor genomes and the potential of personalized neoantigen vaccines. I. Introduction Immunotherapy has fundamentally revolutionized cancer treatment, capitalizing on our growing understanding of complex immune interactions. C.. 2023. 7. 26. What is the immune checkpoint blockade? Discover the power of immune checkpoint blockade in cancer treatment. Learn how this innovative therapy activates the immune system to fight cancer cells efficiently. Find out about the key immune checkpoints targeted and the potential benefits of this approach in enhancing patient outcoming Immune checkpoint blockade Immune checkpoint blockade refers to a therapeutic approach in cancer treatmen.. 2023. 7. 13. The various Cells in Our Blood: Tiny Guardians Protecting Our Health What cells are in our blood? we will explore the various blood cells that play important roles in protecting our health. What kinds of cells are in the blood? 1. Hematopoietic stem cell 2. Common myelolid progenitor 3. Common lymphoid progenitor 4. Megakaryocyte 5. Eosinophil 6. Basophil 7. Erythrocytes 8. Monocyte 9. Dendritic cell - cDendritic cell 1, cDendritic cell 2, plasmacytoid Dendritic .. 2023. 6. 28. 랜덤포레스트 분석 실무에서 랜덤포레스트 분석을 하는 경우는 다음과 같습니다. 연구자들이 진행한 데이터 세트 하나가 있고, 이 연구 데이터의 결과를 다시 확인해 줄 다른 유전자 데이터 세트 하나가 있습니다. 쉽게 이해하자면, 본인의 연구 결과가 다른 연구 결과에서도 증명되었으므로 타당하면서 좋은 연구다라고 주장하고 싶을 때 랜덤포레스트 분석을 한다고 생각하면 됩니다. 랜덤포레스트 분석 순서의 이해 1. 연구 데이터세트와 라벨 할당 unsupervised clustering 방식으로 데이터세트를 그룹핑(grouping) 합니다. 그룹핑 후, 5개의 그룹으로 나누었으면 각 그룹에 라벨을 할당 (cluster1, cluster2, cluster3, cluster4, cluster5)합니다. 2. 랜덤포레스트(random fore.. 2023. 6. 7. What is the micelles? A micelle is a tiny particle or aggregate of moleculess in a colloidal solution. Colloidal solutions are systems consisting of a dispersed phase (micelles) and a continous phase (the solvent). The structure of Micelles Micelles are typically formed surfactant molecules, which are molecules that have both hydrophilic (water-loving) and hydrophobic (water-fearing) parts. The surfactant molecules o.. 2023. 5. 23. How to organize a good review paper It is not easy to write a review paper. You read many articles related to your research's topic, but your memory is limited. In spite of many recording and saving methods, the best way is to write a review paper about your topic. Let us analyze how to structure a good review paper. How to organize a review paper In this post, I will use the EGFR family as an example. Since we are going to write .. 2023. 5. 3. Brief Overview about EGFR family As researcher about life science, we are very familiar with the EGFR gene. However, someone suddenly ask me, What is the EGFR? Can you answer about that? This post will give you a brief overview of EGFR. What is the EGFR family? The full name of EGFR is Epidermal Growth Factor Receptor, and it has family. The EGFR family is a group of receptor tyrosine kinases that play important roles in the re.. 2023. 4. 28. How to differentiate between dimerization and interaction of Transcription Factors Is not there a confusion between dimerization and interaction of Transcription Factors? Someone asks me every day, have you found a protein that transcription factors binds to? or Have you found a protein that it dimerizes? The terms "dimerization (forming a dimer)" and "interaction" have different meaning when discussing transcription factors Dimerization This terms refers to the process where .. 2023. 4. 28. A must-read list of journals to write good reviews Every scientifc journal in the world has an infinite value. The journal list mentioned in this post is a subjective view. The indispensible Journal lists for writing fine review paper in Cancer Research Nature Cancer Nature Reviews Cancer Nature Reviews Immunology Nature Metabolism Nature Medicine Nature Reviews Molecular Cell Biology Nature Reviews Clinical Oncology Nature Reviews Drug Discrove.. 2023. 4. 13. Harnessing Acellular Slime Mold for Tumor-Targeting Therapies: Challenge and Oppotunities Let us explore the interesing properties of the protist Physarum polycephalum, sometimes known as the acellular slime mold or myxomycete. I suggest a hypothesis related to use genetically engineered Physarum polycephalum to target and attack tumors. 1. Introduction to Physarum Polycephalum Physarum polycephalum, also known as acellular slime mold or myxomycete, is a protist characterized by its .. 2023. 4. 10. Medulloblastoma Subgroups and the Role of WNT signaling Mutations in CTNNB1 and APC Genes Explore the classification of Medulloblastoma into four subgroups and understand the impact of CTNNB1 and APC gene mutations driving constitutive WNT signaling in the context. Medulloblastoma Subtypes: Wnt Signaling Mutaions in CTNNB1 and APC genes We know that Medulloblastoma was divided into four subgroups: WNT, SHH, Group3, and Group4. In the context of molecular mutations, the somatic CTNNB1.. 2023. 4. 6. What is the meaning of trajectory? Let us explore the meaning of "trajectory" as it relates to the progression, direction, or course of biological phenomena, cell, tissues, or system over time. The meaning of tracjectory In the context of biology and neuroscience, a "trajectory" typically refers to the developmental or functional pathway of a process or a series of events. It describes the progression, direction, or course of spe.. 2023. 4. 6. Chronological Order: The History of artifical cell research Recently, I have developed a keen interest in the fascinating field of artificial cells, which involves the creation of cell-like structures that can mimic the functions of natural cells. To deepen my understading of the cutting-edge area of research, I have complied a chronological histroy of artificial cell research. Pioneers of Artificial Cell Research Here is a chronological overview of the .. 2023. 4. 3. Harnessing the Power of Microbes to Combat Climate Change: Strategies and Benefits Discover how microbes play a crucial role in mitigating climate change through carbon sequestration. Learn about strategies that leverage microbial power for a more sustainable future. Exploring the Power of Microbes for Carbon Sequestration and a Sustainable Future Microbes are microscopic organisms that play a crucial role in maintaining the Earth's ecosystems. They help regulate the carbon cy.. 2023. 4. 3. The Role of Microtubules in Cell Division and Cancer: A Comprehensive Analysis Discover microtubules' essential function in cell division, their link to cancer, and the potential for targeted therapies in treating the disease. Introduction to Microtubules Microtubules are essential components of the eukaryotic cytoskeleton, responsible for maintaining cell shape, enabling cell motility, and participating in essential cellular processes such as cell division and intracellul.. 2023. 4. 1. Comprehensive Guide to DNA Sequencing: Technologies, Applications, and Future Prospects Discover the evolution of DNA sequencing, from Sanger to next - and third-generation technologies, and delve into their applications and future developments. Table of Contents Introduction History of DNA Sequencing Sanger Sequencing Next-Generation Sequencing (NGS) Third-Generation Sequencing Comparing Sequencing Technologies Applications of DNA Sequencing Future Prospects Conclusion Introductio.. 2023. 3. 31. The Comprehensive Guide to Understanding Resveratrol's Role in Cancer Prevention and Treatment Explore the potential of resveratrol in cancer prevention and treatment, uncovering its anti-cancer mechanisms and addressing current challenges. Table of Contents Introduction to Resveratrol Anti-Cancer Mechanisms of Resveratrol Resveratrol in Cancer Prevention Resveratrol in Cancer Treatment Challenges and Future Perspectives Conclusion Introduction to Resveratrol Resveratrol is a naturally oc.. 2023. 3. 31. History of Molecular Biology Discover the history of molecular biology, from groundbreaking milestones to innovations that revolutionized science and medicine. Join our journey! I. Introduction A. Definition of molecular biology B. Importance and relevance of the field II. Early discoveries and milestones A. 19th century: Gregor Mendel's work on genetics B. 20th century: DNA structure and the role of nucleic acids 1. Freder.. 2023. 3. 29. 이전 1 다음
